Part:BBa_T2007:Design

c-Myc head domain (EQKLISEEDL)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This tail domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts.

An ATG start codon was used followed by a AAA second codon. AAA is the preferred codon for the second codon position in E. coli.

Source

This is a synthetic sequence.

References

<biblio>

1. Kolodziej P.A. Kolodziej and R.A. Young. Epitope tagging and protein surveillance. Methods Enzymol 1991; 194():508-519. doi:10.1016/0076-6879(91)94038-E
2. Terpe pmid=12536251

</biblio>